微管蛋白由α和β微管蛋白组成,在进化过程中其氨基酸和核苷酸序列具有相当高的保守性。微管蛋白基因属多基因家族,并因种而异。本文通过对利什曼原虫基因组DNA的限制性酶切片段长度的多态分析(RFLP)及Southern杂交,对新旧大陆利什曼原虫微管蛋白基因构成的异同作了比较。 用一系列不同限制性内切酶对新大陆墨西哥利什曼原虫及巴西利什曼原虫两组复合体的基因组DNA消化后电泳分离,经双向 Tubulin consists of α and β tubulin, and its amino acid and nucleotide sequences are highly conserved during evolution. Tubulin gene is a multi-gene family, and vary by species. In this paper, the similarities and differences of the tubulin gene of Leishmania newtoni were compared by the restriction fragment length polymorphism analysis (RFLP) and Southern blot of Leishmania genomic DNA. A series of different restriction endonucleases were used to digest the genomic DNA of two sets of complexes of Leishmania mexicana and Leishmania braziliensis,