目的：评价附睾取精术结合卵浆内单精子注射技术治疗阻塞性无精症所致不育症的疗效。方法：对２４例精液常规检查无精症的男性不育患者之妻子，进行超排卵治疗３０个周期。获卵当日同时从患者阴囊行微切口附睾取精液，或经皮附睾穿刺抽吸术获得活动精子，进行卵浆内注射。精子准备方法可从常规的Ｍｉｎｉｐｅｒｃｏｌ法简化为作者自创的“Ｎ”字游出法。结果：３０个治疗周期共获卵３７８个，其中２９８个（占７８．８％）为减数分裂中期Ⅱ的卵子，可行显微精子注射，受精率和卵裂率分别为５５．７％和９４．６％；每周期平均移植的胚胎数３．６个。在所有治疗周期中共妊娠９例，其中流产１例，已分娩３例，继续妊娠５例，妊娠率为３０．０％／周期（９／３０）。结论：精路阻塞的无精症不育患者，只要获得活动精子，结合卵浆内单精子注射，仍然有机会获得妊娠。 Objective: To evaluate the efficacy of epididymal sperm injection combined with intracytoplasmic sperm injection in the treatment of infertility caused by obstructive azoospermia. Methods: Twenty-four female infertile men who had azoospermia were routinely examined for 30 cycles of superovulation. On the day of ovulation, sperm from the epididymis of patients with scrotal micro-incision or percutaneous epididymal aspiration were used to obtain active sperm for intra-oocyte injection. Sperm preparation methods can be simplified from the conventional Minipercol method to the author’s own “N” word out of the law. Results: A total of 378 eggs were obtained during the 30 treatment cycles, of which 298 (78.8%) were metaphase Ⅱ eggs. The injection of micro spermatozoa was feasible. The fertilization rate and cleavage rate were 55.7% and 94.6%; the average number of embryos transferred per cycle was 3.6. In all treatment cycles, 9 cases of pregnancy were common, including 1 abortion, 3 cases of childbirth and 5 cases of pregnancy. The pregnancy rate was 30.0% / cycle (9/30). CONCLUSIONS: Azoospermic infertility patients with clogged seminoma, as long as the activity of sperm, combined with intracytoplasmic sperm injection, still have the opportunity to get pregnant.