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将毛细管区带电泳／前沿分析技术用于测定碱性药物ｖｅｒａｐａｍｉｌ（ＶＥＲ）和ｐｒｏｐｒａｎｏｌｏｌ（ＰＲＯ）与人血清白蛋白（ＨＳＡ）平衡体系中结合参数的研究。通过压力进样将药物白蛋白混合液引入石英毛细管电泳柱（３２ｃｍ×５０μｍｉ．ｄ．，聚丙烯酰胺涂渍柱），在ｐＨ值为７．４、离子强度为０．１７的磷酸缓冲液及运行电压为１０ｋＶ的条件下，未结合的碱性药物的浓度可以通过电泳平台峰的高度定量，并且具有良好的线性关系（相关系数ｒ＝０．９９９）。在数据处理过程中成功的采用非线性回归、Ｓｃａｔｃｈａｒｄ方程和Ｋｌｏｔｚ方程３种评价方法，得到了一致的结果。得到的ＶＥＲ和ＰＲＯ与ＨＳＡ的结合参数：结合位点分别是１．２１～１．３０和０．５４～０．６２，结合常数分别是１６９０～１７９０和１３９０～１６９０
Capillary Zone Electrophoresis / Forefront Analysis was used to study the binding parameters of the verapamil (VER) and propranolol (PRO) and human serum albumin (HSA) equilibrium systems. The drug albumin mixture was introduced into the quartz capillary electrophoresis column (32cm × 50μmi.d., Polyacrylamide coated column) by pressure injection at a pH of 7.4, the ionic strength of 0.17 phosphate buffer and Under the condition of operating voltage of 10 kV, the concentration of unbound basic drug can be highly quantified by the height of the electrophoresis platform and has a good linear relationship (correlation coefficient r = 0.999). In the process of data processing, three kinds of evaluation methods, non-linear regression, Scatchard equation and Klotz equation, were successfully adopted, and the same results were obtained. The binding parameters of VER and PRO obtained with HSA were as follows: the binding sites were 1.21-1.30 and 0.54-0.62 respectively, and the binding constants were 1690-1790 and 1390-1690 respectively