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目的探讨更有效的乙型肝炎病毒(HBV)的检测方法,避免临床漏诊。方法选择明确乙型肝炎病毒感染者40例,所有患者均行电化学发光法和酶联免疫吸附试验(ELISA)检测,比较2种方法测定HBV-DNA定量情况及分析HBsAg阳性率差异。结果电化学发光法检查出HBV-DNA超过105拷贝/ml者显著多于ELISA(P<0.05),ELISA阳性率为62.5%,电化学发光法阳性率为82.5%,电化学发光法阳性率显著高于ELISA(P<0.05)。结论化学发光法定量检测HBV-DNA可以减少乙肝患者漏诊。对低浓度乙肝表面抗原(HBsAg)携带者,特别是医务工作者及患者家属等的早期预防有重要的现实意义。
Objective To explore a more effective method for the detection of Hepatitis B virus (HBV) and avoid clinical misdiagnosis. Methods Forty patients with hepatitis B virus infection were selected. All patients were examined by electrochemiluminescence and enzyme-linked immunosorbent assay (ELISA). Two methods were compared to determine the quantitative of HBV-DNA and analyze the difference of HBsAg positive rate. Results Electrochemiluminescence assay showed that the positive rate of HBV-DNA was higher than that of ELISA (P <0.05) by more than 105 copies / ml, the positive rate of ELISA was 62.5%, the positive rate of electrochemiluminescence was 82.5%, and the positive rate of electrochemiluminescence Higher than ELISA (P <0.05). Conclusion Chemiluminescence quantitative detection of HBV-DNA can reduce the missed diagnosis of hepatitis B patients. The low-level hepatitis B surface antigen (HBsAg) carriers, especially medical workers and their families and other early prevention has important practical significance.