采用微透析结合高效液相色谱法建立了一种研究金属离子与蛋白质竞争结合作用的方法.采用Cu2+,Ni2+,Cd2+,Zn2+与人血清蛋白（human　serum　albumin,HSA）为模型化合物,通过各种离子之间的竞争结合的研究表明:在HSA上Cu2+和Ni2+有共同的第一结合位点,Cd2+和Zn2+有共同的第一结合位点,它们在HSA上有一多离子的共同结合位点.本方法具有通用、快速、方便等特点,可用于无理想谱学探针的蛋白质-金属离子相互作用体系的研究. A method to study the competitive binding between metal ions and proteins was established by using microdialysis combined with high performance liquid chromatography (HPLC) .Methods: Cu2 +, Ni2 +, Cd2 +, Zn2 + and human serum albumin (HSA) The competitive binding between ions shows that Cu2 + and Ni2 + have a common first binding site on HSA, and Cd2 + and Zn2 + have a common first binding site, which has a common binding site for multiple ions on HSA The method is universal, rapid and convenient, and can be used to study the protein - metal ion interaction system of nonideal spectrum probe.