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[目的]用编码单纯疱疹病毒Ⅰ型(HSV-1)糖蛋白B的pDNA转染小鼠,研究基因疫苗治疗感染性疾病的有效性,并分析免疫反应.[方法]采用血管内注射方法,将含有不同剂量、不同pDNAs的生理盐水注入Balb/c小鼠体内,1周后行再次免疫,观察以不同组合pDNA进行免疫的实验组和对照组小鼠被致死剂量HSV-1感染后生存率间的差异.通过潜伏感染的检测、血清中细胞因子水平的测定、细胞毒性反应的测定及中和实验评价免疫效果.[结果]接种pG.gB或接种pGEG.gB的小鼠,与相应对照组比较,生存率和诱导产生中和抗体的效价与剂量呈正相关,pGEG.gB免疫组对P815.gB细胞有显著的细胞毒性作用,pGEG.gB免疫小鼠的脾细胞增殖反应显著强于pG.gB免疫的小鼠;pG.gB和pGEG.gB免疫后的小鼠血清中IL-12和IFN-γ水平均显著升高.[结论]裸pDNA的经小鼠尾静脉免疫可诱导HSV-1感染小鼠的体液和细胞免疫应答,对小鼠HSV-1急性感染有明显的预防和治疗作用,且含有EBNA1和oriP的EBV质粒载体明显增强CTL活性和增殖反应.
[Objective] The purpose of this study was to investigate the effectiveness of gene vaccines in the treatment of infectious diseases by transfecting mice with pDNA encoding herpes simplex virus type 1 (HSV-1) glycoprotein B. [Method] The intravascular injection Balb / c mice were injected with normal saline containing different dosages of different pDNAs and re-immunized one week later. The survival rates of experimental group and control group immunized with different combinations of pDNA were observed after the lethal dose of HSV-1 infection The differences were evaluated by the detection of latent infection, the determination of cytokines in serum, the determination of cytotoxic reaction and the neutralization test. [Results] Compared with the corresponding control group, mice inoculated with pG.gB or inoculated with pGEG.gB , The survival rate and the titer of the induced neutralizing antibody were positively correlated with the dose. The pGEG.gB immunized group had a significant cytotoxic effect on P815.gB cells, and the pGEG.gB immunized mice showed a significantly stronger spleen cell proliferation response. gB immunized mice; the levels of IL-12 and IFN-γin serum of mice immunized with pG.gB and pGEG.gB were significantly increased.Conclusion: The naked pDNA can induce HSV-1 Infected mice humoral and cellular immune responses to mouse HSV- 1 acute infection has a significant preventive and therapeutic effect, and EBV plasmid vector containing EBNA1 and oriP significantly enhance CTL activity and proliferative response.