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目的 确定新分离的冠状病毒与目前流行的严重急性呼吸综合征的病原关系。方法 以用细胞培养法从严重急性呼吸综合征病例标本中分离出的冠状病毒为抗原 ,通过免疫荧光染色及中和试验测定严重急性呼吸综合征患者血清中抗新分离的冠状病毒的抗体 ,分析确定新冠状病毒与严重急性呼吸综合征的病原关系。结果 在临床确诊的 1 1 3份严重急性呼吸综合征患者血清标本中 ,99份检测出有抗新冠状病毒的抗体。 1 0对双份血清检测结果显示 ,后采集的血清抗体效价均比发病初期采集的明显增高 ,最高的升高 1 2 8倍。中和试验结果证明 ,病人血清抗体能够中和新分离的冠状病毒。结论 新分离的冠状病毒与此次流行的严重急性呼吸综合征密切相关 ,是目前流行的严重急性呼吸综合征的主要病原体
Objective To determine the causal relationship between the newly isolated coronavirus and the currently prevailing severe acute respiratory syndrome. Methods The coronavirus isolated from severe acute respiratory syndrome patients by cell culture method was used as antigen. The anti-newly isolated coronavirus antibodies in sera of patients with severe acute respiratory syndrome were determined by immunofluorescence staining and neutralization assay. To determine the new coronavirus and SARS pathogenesis. Results Of the 113 clinically diagnosed sera from patients with SARS, 99 antibodies against the new coronavirus were detected. The results of double sera analysis showed that the serum antibody titers collected after 12 weeks were significantly higher than those collected in the early stage of disease, the highest increase was 128%. The neutralization test results demonstrate that the patient’s serum antibody neutralizes the newly isolated coronavirus. Conclusions The newly isolated coronavirus is closely related to this epidemic of severe acute respiratory syndrome and is currently the major pathogen of severe acute respiratory syndrome