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为了研究筛选出的具有抗氧化活性的2株顶孢霉的发酵液提取物清除自由基的能力,本研究制备了2株顶孢霉的发酵液提取物,比较了其清除O2-·、·OH和DPPH·三种自由基的能力。结果表明:2株顶孢霉发酵液提取物的相对还原力(即总抗氧化活性)均显著高于维生素E;对O2-·自由基的清除率也均高于维生素E,其中,菌株YX015更为高效,在1.0mg/mL浓度时对O2-·自由基的清除率达到49.15%;2菌株清除·OH和DPPH·的能力均显著弱于维生素E,但都高于其自身清除O2-·的能力,在浓度达到1.0mg/mL时,菌株CA022对·OH的清除率为60.43%;菌株YX015对·OH的清除率为83.33%。
In order to study the scavenging ability of two Acremonium fermentation broth extracts with scavenging activity to scavenge free radicals, two Acremonium fermentation broth extracts were prepared in this study. OH and DPPH · three kinds of free radicals. The results showed that the relative reducibility (total antioxidant activity) of the two Acremonium fermentation broth extracts were significantly higher than that of vitamin E; the clearance rate of O2- · radicals was also higher than that of vitamin E, among which strain YX015 Was more efficient. At the concentration of 1.0mg / mL, the scavenging rate of O2- · radical reached 49.15%. The ability of 2 strains to scavenge · OH and DPPH · was significantly weaker than that of vitamin E, but higher than that of O2- · The scavenging capacity of strain CA022 to · OH was 60.43% at the concentration of 1.0 mg / mL. The rate of · OH scavenging by strain YX015 was 83.33%.