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目的探讨痛泻要方对肝郁脾虚型肠易激综合征大鼠内脏敏感性的干预作用。方法采用番泻叶加束缚应激刺激制备肝郁脾虚型肠易激综合征动物模型。予痛泻要方灌胃治疗(西药匹维溴铵对照)。观察大鼠结肠黏膜肥大细胞数目及脱颗粒情况;检测血标本中5-羟色胺(5-HT)、P物质(SP)、降钙素基因相关肽(CGRP)的含量。结果模型对照组大鼠结肠黏膜肥大细胞数目及脱颗粒显著增加;西药组和中药组大鼠5-HT、SP含量显著下降,CGRP含量显著增加,差异均有高度统计学意义(均P<0.01),且有一定的量效关系。结论痛泻要方可能通过减少肥大细胞脱颗粒,降低模型大鼠血清5-HT、血浆SP含量,增加血浆CGRP含量,从而改善胃肠动力并降低内脏敏感性,发挥治疗肠易激综合征的效应。
Objective To investigate the effect of Tongxieyaofang on visceral sensitivity in rats with irritable bowel syndrome due to liver-qi stagnation and spleen deficiency. Methods The senna of irritable bowel syndrome with liver-qi spleen syndrome was induced by senna plus restraint stress. To diarrhea diarrhea side treatment (pinaverium bromide control). The number and degranulation of colonic mucosa in rats were observed. The contents of 5-HT, SP and CGRP in blood samples were determined. Results The number of mast cells and degranulation of colonic mucosa in model control group were significantly increased. The content of 5-HT and SP and the content of CGRP in rats in western medicine group and traditional Chinese medicine group were significantly decreased (both P <0.01 ), And have a certain dose-effect relationship. Conclusion Tong Xie Yao Fang can reduce mast cell degranulation, reduce the model rat serum 5-HT, plasma SP content, increase plasma CGRP content, thereby improving gastrointestinal motility and reduce visceral sensitivity, play a role in the treatment of irritable bowel syndrome effect.