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用蚕豆萎蔫病毒（ＢＢＷＶ）提纯病毒粒子免疫ＢＡＬＢ／ｃ小鼠，取其脾细胞与ＳＰ２／０骨髓瘤细胞融合，用纯化的ＢＢＷＶ病毒对杂交瘤细胞及时筛选，阳性孔经三次有限稀释法克隆，成功获得２株ＢＢ－ＷＶ特异性单克隆抗体的细胞株，分别命名为４Ｄ１１，３Ｇ１２．２株单抗腹水间接ＥＬＩＳＡ效价高达１∶６４００００，抗体类型均为ＩｇＧ１，经Ｗｅｓｔｅｒｎ－ｂｌｏｔｉｎｇ分析表明，２株单抗均是针对ＢＢＷＶ４４．７ｋＤ的外壳蛋白大亚基的特异性抗体．单抗抗原结合位点分析表明４Ｄ１１和３Ｇ１２两单抗作用于同一抗原决定簇
BALB / c mice were immunized with the purified virus particles of broad bean wilt virus (BBWV). The spleen cells were fused with SP2 / 0 myeloma cells. The hybridoma cells were screened by purified BBWV virus. The positive wells were cloned by three limiting dilution , Successfully obtained two BB-WV-specific monoclonal antibody cell lines were named 4D11, 3G12.2 monoclonal antibody ascites indirect ELISA titers as high as 1: 640000, antibody types were IgG1, Western-bloting analysis showed that , Two monoclonal antibodies against BBWV44.7kD coat protein large subunit specific antibodies. Analysis of mAb antigen binding sites revealed that 4D11 and 3G12 mAbs act on the same epitope