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目的 筛选不同转移潜能肝癌细胞株的 microRNA (miRNA)差异表达谱,并预测分析差异表达miRNAs调控的靶基因与功能.方法 提取总RNA进行miRNA芯片分析,通过分析MHCC-97H(高转移)、Hep3B(不转移)两种不同转移潜能肝癌细胞株与正常肝细胞L02比较,以及两种肝癌细胞株相互比较的miRNA差异表达谱,筛选出差异表达明显的miRNAs进行qRT-PCR验证,利用4个靶基因预测软件(TargetScan、miRanda、miRWalk、miRDB)进行靶基因预测,并通过生物信息学分析来探讨候选靶基因的生物学功能.结果 与正常肝细胞 L02相比,肝癌细胞株 MHCC-97H、Hep3B中的 miR-192-5p、miR-215-5p 表达均明显上调,而miR-130a-3p、miR-196a-5p 则明显降低;与不转移肝癌细胞株Hep3B相比,miR-224-5p在肝癌细胞株 MHCC-97H中明显上调,而miR-146a-5p、miR-483-3p、miR-200b-3p则明显降低.qRT-PCR验证结果与芯片结果相一致.结论 MHCC-97H及Hep3B两种转移能力不同的肝癌细胞株的 miRNAs存在差异表达,这些差异表达的miRNAs与肝细胞癌的发生发展、转移侵袭密切相关.“,”Aim To screen the differential microRNA (miRNA) expression profiles of different metastatic po-tential liver cancer cell lines,and predict miRNAs-reg-ulated target genes and their functions. Methods To-tal RNA was extracted and the miRNA expression pro-files were obtained by miRNA microarray chip hybrid-ization. The miRNAs whose expression had significant difference were selected by analyzing the miRNA difference expression profiles of the two different meta-static potential liver cancer cell lines, namely MHCC-97H(high-metastasis) and Hep3B(non-metastasis), which were compared with normal hepatocytes L02 re-spectively. Moreover, we analyzed the miRNA differ-ential expression profile between liver cancer cell lines MHCC-97H and Hep3B. The miRNAs were verified by qPCR and target genes were predicted by four softwares (TargetScan, miRanda, miRWalk, miRDB). To un-derstand the biological functions of predicted target genes, bioinformatics analysis was performed. Results The miRNA microarray results showed that the ex-pression of miR-192-5p and miR-215-5p significantly increased in liver cancer cell lines (MHCC-97H, Hep3B) when compared with normal hepatocytes L02, while miR-130a-3p and miR-196a-5p were significantly reduced; compared with Hep3B, the expression of miR-224-5p markedly increased in liver cancer cell line MHCC-97H, while miR-146a-5p, miR-483-3p and miR-200b-3p were significantly reduced. The re-sults of qRT-PCR were consistent with chip results. Conclusion There are differences of miRNA expres-sion profiles in different metastatic potential liver canc-er cell lines MHCC-97H, Hep3B, and they may par-ticipate in regulating the development and invasion of hepatocellular carcinoma.