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随机扩增的多态性DNA(RAPD)分析是近年来发展起来的以多聚酶链式反应(PCR)为基础的一项新方法.它通过短引物(通常含9—10碱基)和模板间在较低的退火温度下配对,引发在基因组若干位点的DNA扩增,获得产物.RAPD技术快速,易行,只需少量的DNA,因而在许多领域得到应用,条锈病是我国北方麦区危害较为严重的病害之一,尤其是近年随着新的生理小种条中28,29的出现,使得原来抗条锈的洛夫林10,13号等1B/1R代换系或易位系的抗性逐渐丧失,因而急需新的抗条锈病品种.本文报道利用RAPD方法对1个新的抗条锈病的小麦-黑麦易位系进行了分析,同时还讨论了RAPD技术的某些局限性.
Random amplified polymorphic DNA (RAPD) analysis is a new method based on polymerase chain reaction (PCR) which has been developed in recent years. It uses short primers (usually 9-10 bases) Pairing at a lower annealing temperature initiates DNA amplification at several loci in the genome to obtain the product. The RAPD technique is fast, easy and requires little DNA and is therefore used in many fields, Especially in recent years, with the emergence of new physiological races 28 and 29, the original 1B / 1R substitution lines or translocation lines such as Lovrin 10 and 13, which are resistant to stripe rust, The new resistance to stripe rust was urgently needed.In this paper, a new RAPD-resistant wheat-rye translocation line was analyzed and some limitations of RAPD technique were also discussed Sex.