论文部分内容阅读
目的建立裸鼠原位肝癌耐药模型。方法培养肝癌细胞系HepG2,建立裸鼠的皮下肿瘤,形成“供瘤鼠”。开腹直视下将瘤块种植于裸鼠的肝包膜下建立原位肝癌模型,通过表阿霉素间歇腹腔化疗,建立裸鼠原位肝癌耐药模型。用体检、B超、CT、剖腹探查监测肝内瘤块生长情况。用逆转录聚和酶链反应(RT-PCR)和免疫组织化学方法检测肿瘤耐药基因mdrl-mRNA和p-gp蛋白的表达。结果(1)模型建立无手术死亡(0/25),种植成瘤率为88%(22/25),补种3例全部成功,耐药诱导成功率为80%(16/20);(2)诱导组mdrl-mRNA和P-gp蛋白的表达均明显高于对照组,分别约是对照组的23倍和13倍。结论成功地建立了与临床肝癌相似的裸鼠原位肝癌耐药模型,为进一步研究肝癌多药耐药基因的诊断和逆转提供了良好的动物平台。
Objective To establish a model of orthotopic liver cancer in nude mice. Methods HepG2 cells were cultured in vitro and subcutaneous tumors of nude mice were established to form “donor mice”. Under open vision, the lumps were planted in the hepatic capsule of nude mice to establish the model of orthotopic liver cancer. The model of orthotopic liver cancer in nude mice was established by intermittent intraepithelial chemotherapy with epirubicin. With physical examination, B ultrasound, CT, laparotomy to detect intrahepatic lump growth. The expression of mdrl-mRNA and p-gp protein were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. Results (1) There was no surgical death (0/25) in the model, the rate of tumorigenesis was 88% (22/25), all three cases were successful, and the success rate of drug resistance induction was 80% (16/20) 2) The expression of mdrl-mRNA and P-gp protein in induced group were significantly higher than those in control group, which were about 23 times and 13 times that in control group respectively. Conclusion The model of orthotopic liver cancer in nude mice which is similar to clinical liver cancer has been successfully established, which provides a good animal platform for the further study on the diagnosis and reversal of multidrug resistance gene in liver cancer.