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以[_3H]DHA为放射配基,比较了人、家兔和大鼠血淋巴细胞β-受体与配基结合的若干参数。结果表明,不同种属间淋巴细胞β-受体密度存在差异,依次为家兔(n=9)104.5±19.8 fmol/10~6细胞>大鼠(n=5)68.8±12.3 fmol/10~6细胞>人(n=13)56±8.65 fmol/10~6细胞,(X±SD)。但是三者平衡解离常数Kd值相近,分别为8.39±2.76nM,6.34±2.99nM,6.96±1.62nM(X±SD)。Hill系数均极接近于1。提示,家兔、大鼠和人血淋巴细胞与β受体配基的结合过程属同一类型。兔和大鼠疾病模型中淋巴细胞β受体变化的资料对人类疾病时β受体作用的研究有一定的参考价值。
Using [_3H] DHA as a radioligand, we compared the binding of beta-receptors to ligands in human, rabbit and rat blood lymphocytes. The results showed that there were differences in β-adrenergic lymphocyte density among different species, which were as follows: 104.5 ± 19.8 fmol / 10 ~ 6 cells from 68.8 ± 12.3 fmol / 10 ~ 6 cells> human (n = 13) 56 ± 8.65 fmol / 10-6 cells, (X ± SD). However, the Kd values of equilibrium dissociation constants of the three were similar, which were 8.39 ± 2.76nM, 6.34 ± 2.99nM and 6.96 ± 1.62nM (X ± SD) respectively. Hill coefficients are very close to 1. Tip, rabbit, rat and human blood lymphocytes and β receptor ligand binding process are the same type. Data on changes of lymphocyte beta receptors in rabbit and rat models of disease have some reference value for the study of beta receptor function in human diseases.