按照我们报道的方法进行人脐静脉内皮细胞的体外长期传代培养。所培养的细胞经间接免疫荧光法检测Ⅷ.RAg及透射电镜观察胞质中Weible-Palade小体而证实为血管内皮细胞。在每次传代培养前,收集条件培养液,置-20℃冰箱内保存备用。用放射免 Long-term subcultures of human umbilical vein endothelial cells were performed in vitro as we reported. The cultured cells were detected by indirect immunofluorescence Ⅷ.RAg and transmission electron microscopy of the cytoplasm of Weible-Palade bodies confirmed vascular endothelial cells. Before each subculture, the conditioned medium was collected and stored at -20 ° C for later use. With radio-free