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目的:建立测定小鼠血浆中银杏酸的高效液相色谱法。方法:样品预处理以乙腈沉淀蛋白。采用SinoChrom ODS-AP C18色谱柱(250mm×4.6mm,5μm),甲醇-3%醋酸(92∶8)为流动相,流速1.0mL·min-1,检测波长310nm。结果:银杏酸在0.25～50μg·mL-1范围内线性关系良好(r=0.9998),最低检测限为0.08μg·mL-1。提取回收率为87.0%～90.2%(n=5),相对回收率为95.8%～100.1%(n=5),日内RSD≤5.8%(n=5),日间RSD≤9.5%(n=5)。结论:该法灵敏度高,重现性好,精密度和准确性好,适于银杏酸的药代动力学研究。
Objective: To establish a HPLC method for determination of ginkgolic acid in mouse plasma. Methods: Samples were pretreated with acetonitrile to precipitate proteins. Using SinoChrom ODS-AP C18 column (250mm×4.6mm, 5μm), methanol-3% acetic acid (92:8) as mobile phase, flow rate 1.0mL·min-1, detection wavelength 310nm. RESULTS: The linearity of ginkgolic acid was good in the range of 0.25-50μg·mL-1 (r=0.9998). The detection limit was 0.08μg·mL-1. The recoveries were 87.0%-90.2% (n=5), relative recoveries were 95.8%-100.1% (n=5), intraday RSD was ≤5.8% (n=5), and RSD was ≤9.5% during the day (n= 5). Conclusion: The method has high sensitivity, good reproducibility, good precision and accuracy, and is suitable for the pharmacokinetic study of ginkgo acid.