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目的探讨基质金属蛋白酶-8(MMP-8)对角膜的作用。方法选取健康C57BL/6J小鼠15只,右眼角膜基质内注射10μL MMP-8作为实验组,左眼给予等量生理盐水作为对照组。于0、4、8h使用双光子显微镜二次谐波成像技术对活体小鼠角膜逐层扫描,Imaris软件对所得图像三维重建,计算图像信号强度。4、8h在裂隙灯下评价角膜混浊度。8h角膜取材,测定各角膜羟脯氨酸水平。结果 0h实验组及对照组小鼠角膜基质纤维信号强度分别为89.7±11.2、85.3±7.0,4h分别为14.5±3.4、46.6±14.0,8h分别为11.0±4.6、34.6±12.5,4h及8h,实验组较对照组角膜基质信号强度明显降低(P<0.05);4h及8h,实验组较对照组角膜明显混浊(P<0.05);8h测得实验组与对照组角膜羟脯氨酸水平分别为(0.433±0.090)、(0.590±0.133)μg/mg,实验组明显低于对照组(F=7.193,P=0.014)。结论 MMP-8对小鼠角膜基质胶原有明显的降解破坏作用,导致角膜透明度下降。
Objective To investigate the effect of matrix metalloproteinase-8 (MMP-8) on the cornea. Methods Fifteen healthy C57BL / 6J mice were injected with 10 μL of matrix metalloproteinase-8 (MMP-8) in the cornea of the right eye, and the same volume of normal saline was given to the left eye as the control group. At 0, 4, and 8 h, the cornea of living mice was scanned by two-photon microscopy second harmonic imaging technique. The image was reconstructed by Imaris software to calculate the image signal intensity. 4,8h under the slit lamp evaluation of corneal opacity. 8h corneal access, determination of the corneal hydroxyproline levels. Results The signal intensity of corneal stromal fibers in the experimental group and the control group was 89.7 ± 11.2, 85.3 ± 7.0 and 4 h, respectively, at 14 hours, respectively, 11.0 ± 4.6 and 34.6 ± 12.5, 4 h and 8 h, respectively, The signal intensity of corneal stroma in the experimental group was significantly lower than that in the control group (P <0.05). At 4h and 8h, the cornea of the experimental group was significantly cloudy (P <0.05); the levels of corneal hydroxyproline in the experimental group and the control group were respectively (0.433 ± 0.090) and (0.590 ± 0.133) μg / mg respectively. The experimental group was significantly lower than the control group (F = 7.193, P = 0.014). Conclusion MMP-8 has obvious degradation and destruction effect on corneal collagen of mice, resulting in the decrease of corneal transparency.