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立了一种测定栀子中的栀子甙含量的高效液相色谱分析方法 .在Kromasil-C18(4 .6mm× 2 5 0mm ,5 μm)柱上进行分析 ,流动相为水 -甲醇 -醋酸 (80∶2 0∶0 .1,V V) ,流速为 0 .8mL min ,紫外检测波长为 2 4 0nm ,0 .0 2AUFS ,栀子甙的平均回收率为 99.35 %,相对标准偏差RSD为 1.2 8(n =6) ,这种方法测定栀子甙准确 ,快捷 ,简单 .
A high performance liquid chromatographic method was developed for the determination of geniposide in Gardenia jasminoides.Analysis was performed on a Kromasil-C18 (4.6 × 250 mm, 5 μm) column using water-methanol-acetic acid (80:20:01, VV), the flow rate was 0.8mL min, UV detection wavelength of 240nm, 0.2AUFS, the average recovery of Geniposide was 99.35%, the relative standard deviation of RSD was 1.2 8 (n = 6), this method for determination of Geniposide accurate, fast and simple.