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试验采用鲍氏志贺氏菌分离株对3日龄SPF雏鸡进行攻毒,观察其肝、脾、法氏囊等组织的病理变化,应用常规石蜡切片和H.E染色,进行组织结构观察。结果表明:在所有剖检鸡只中脾和法氏囊均无明显眼观病变,而肝出现了出血、充血、周边坏死、色发黄、质变脆等病变;组织学病变主要有出血、淤血、变性、坏死,法氏囊大面积上皮细胞脱落、变性、坏死及炎性浸润等病变。可见此菌对鸡有极强的致病性。
The experiment used Shigella blastaniella isolates to challenge the 3-day-old SPF chickens, and observed the pathological changes of the liver, spleen and bursa of Fabricius. The histological observation was performed by routine paraffin sections and H.E staining. The results showed that there was no obvious pathological change in the spleen and bursa of all the chickens examined, and the liver showed hemorrhage, congestion, peripheral necrosis, yellowish color and brittle quality. The main histological lesions were hemorrhage, congestion , Degeneration, necrosis, large area of bursa epithelial cells shedding, degeneration, necrosis and inflammatory infiltration and other lesions. This bacteria can be seen on the chicken has a strong pathogenicity.