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目的将H5N1亚型禽流感病毒(AIV)鼻腔接种BALB/c小鼠,动态观察小鼠每个时期主要器官组织的病理变化。方法将100μlH5N1亚型禽流感病毒原液滴入经麻醉后BALB/c小鼠鼻腔,观察14d,每天取材1次,固定、包埋、切片后HE染色观察各组织病理变化。结果 H5N1亚型禽流感病毒感染BALB/c小鼠后,产生一系列与禽流感病毒感染有关的动态病理改变:第1～2天(前驱期),肺轻微出血、水肿、炎性细胞浸润;第3～7天(发作期),肺损伤逐渐严重,大量出血、炎性细胞浸润、严重水肿、淤血、肺泡实变塌陷或者气肿;第8～14天(恢复期),各种损伤逐渐减轻,出血渗出减少,水肿减轻,肺间质出现纤维化而趋于恢复正常。肝、肾、脑出现病理改变。结论通过动态观察病理,弄清了禽流感病毒每个时期在BALB/c小鼠体内造成的病理损伤。
Objective To inoculate BALB / c mice with H5N1 subtype of avian influenza virus (AIV) nasally and observe the pathological changes of major organs and tissues in each period of mice dynamically. Methods 100μl H5N1 subtype avian influenza virus solution was instilled into the nasal cavity of anesthetized BALB / c mice and observed for 14 days. The samples were harvested once a day, fixed, embedded and stained for histopathological changes. Results A series of dynamic pathological changes associated with avian influenza virus infection were found in BALB / c mice infected with H5N1 subtype of avian influenza virus: slight hemorrhage, edema and infiltration of inflammatory cells on days 1 to 2 (prodromal stage) From the third to the seventh days (onset), the lung injury was gradually serious, massive bleeding, inflammatory cell infiltration, severe edema, congestion, alveolar solidification collapse or emphysema; the first 8 to 14 days (recovery) Reduce bleeding exudation reduced edema reduced interstitial fibrosis and tends to return to normal. Liver, kidney, brain pathological changes. Conclusions The pathological changes of BALB / c mice during each period of the bird flu virus were clarified through dynamic observation of the pathology.