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目的:探讨miR-424-5p对宫颈癌放射敏感性的影响及作用机制。方法:RT-qPCR检测miR-424-5p在宫颈癌组织和Hela细胞中表达；流式细胞术检测Hela细胞凋亡率；CCK-8检测Hela细胞增殖活性；蛋白印迹法检测Hela细胞中蛋白表达水平。结果:相较于正常组织和细胞，宫颈癌组织和Hela细胞中miR-424-5p表达量降低(1.03∶0.88，n P<0.01；1.00∶0.75，n P<0.001)。过表达miR-424-5p会抑制经放射处理后Hela细胞增殖活性(n P<0.01)，同时增加放射处理后Hela细胞凋亡率(24.82%∶49.94%，n P<0.001)。过表达miR-424-5p会抑制HMGA1表达(1.01∶0.63，n P<0.01)，miR-424-5p会直接作用HMGA1进而影响宫颈癌放射敏感性。n 结论:miR-424-5p通过直接靶向HMGA1提高宫颈癌放射敏感性。“,”Objective:To investigate the effect of miR-424-5p on radiosensitivity and its mechanism in cervical cancer patients.Methods:The expression levels of miR-424-5p in the cervical cancer tissues and Hela cells were detected by RT-qPCR. The apoptosis rate of Hela cells was determined by flow cytometry. The proliferation activity of Hela cells was detected by CCK-8 assay. The protein expression levels in Hela cells were measured by Western blot.Results:Compared with normal tissues and cells, the expression level of miR-424-5p was significantly down-regulated in the cervical cancer tissues and Hela cells (1.03 n vs. 0.88, n P<0.01; 1.00n vs. 0.75, n P<0.01). Overexpression of miR-424-5p significantly inhibited the proliferation activity of Hela cells after radiation treatment (n P<0.01), and significantly increased the apoptosis rate of Hela cells after radiation treatment (24.82%n vs. 49.94%, n P<0.001). Overexpression of miR-424-5p inhibited HMGA1 expression (1.01n vs. 0.63, n P<0.01). miR-424-5p directly affected HMGA1, thereby impacting the radiosensitivity of cervical cancer radiotherapy.n Conclusion:miR-424-5p can improve the radiosensitivity of cervical cancer radiotherapy by directly targeting HMGA1.