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目的:研究PCO—Pin,Nic,Lem及RP对VMSC内[Ca~(2+)]_i的改变及其可能机制。方法:VSMC加入Fura-2 AM 2.5μmol·L~(-1)37℃下孵育50min,[Ca~(2+)]_i用荧光分光光度计检测。结果:4种PCO能较弱地抑制K~+30 mmol·L~(-1)诱导的[Ca~(2+)]_i增加,但明显抑制ATP 0.1mmol·L~(-1)诱导的[Ca~(2+)]_i峰相及持续相增加,且呈剂量依赖性。格列苯脲完全阻断Pin,Lem及RP的作用,只部分抑制Nic的作用。无钙液中先给4种PCO,能显著抑制ATP诱导的[Ca~(2+)]_i峰相增加。结论:4种PCO均抑制ATP诱导的[Ca~(2+)]_i增加,此作用与减少细胞外钙内流及细胞内钙释放有关。
OBJECTIVE: To investigate the changes of [Ca ~ (2 +)] _i and their possible mechanisms by PCO-Pin, Nic, Lem and RP in VMSCs. Methods: VSMCs were incubated with Fura-2 AM 2.5μmol·L -1 at 37 ℃ for 50min. [Ca 2+] i was detected by fluorescence spectrophotometer. Results: Four kinds of PCO could weaken the increase of [Ca ~ (2 +)] _i induced by K ~ +30 mmol·L ~ (-1), but significantly inhibited the increase of ATP induced by 0.1 mmol·L ~ (-1) [Ca ~ (2 +)] _i peak phase and sustained phase increased in a dose-dependent manner. Glibenclamide completely blocked the action of Pin, Lem and RP and only partially inhibited the effect of Nic. Pretreatment with 4 PCO in calcium-free solution significantly inhibited the increase of [Ca ~ (2 +)] _i peak induced by ATP. CONCLUSION: Four kinds of PCO can inhibit the increase of [Ca ~ (2 +)] _i induced by ATP, which is related to the decrease of extracellular calcium influx and intracellular calcium release.