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[Objective] This study aimed to screen and identify tobacco mutants resistant to potato virus Y( PVY),thus laying the foundation for obtaining PVY resistance genes. [Method]At seedling stage,tobacco mutant materials were inoculated with PVY virus and preliminarily screened by naked-eye observation. Enzyme-linked immunosorbent assay( ELISA),real-time fluorescence quantitative PCR and test strip assay were performed to further identify the pre-screened PVYresistant seedlings. [Result] In 2011,two highly PVY-resistant tobacco mutants( MZE2-15 and MZE2-16) and three PVY-tolerant mutants( MZE2-70,MZE2-207 and MZE2-228) were obtained,which were further screened and identified in 2012. According to the results,tobacco mutant materials MZE2-407 and MZE2-428 were susceptible to PVY; mutant materials MZE2-16 and MZE2-15 were resistant to PVY. [Conclusion]This study provide theoretical basis for the control of tobacco PVY disease.
[Objective] This study aimed to screen and identify tobacco mutants resistant to potato virus Y (PVY), laid in the foundation for obtaining PVY resistance genes. [Method] At seedling stage, tobacco mutant materials were inoculated with PVY virus and preliminarily screened by naked-eye observation. Enzyme-linked immunosorbent assay (ELISA), real-time fluorescence quantitative PCR and test strip assay were performed to further identify the pre-screened PVYresistant seedlings. [Result] In 2011, two highly PVY- MZE2-15 and MZE2-16) and three PVY-tolerant mutants (MZE2-70, MZE2-207 and MZE2-228) were obtained, which were further screened and identified in 2012. According to the results, tobacco mutant materials MZE2-407 and MZE2-428 were susceptible to PVY; mutant materials MZE2-16 and MZE2-15 were resistant to PVY. [Conclusion] This study provides theoretical basis for the control of tobacco PVY disease.