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取HBsAg阳性献血员的血清,选其中不含Dane颗粒或e抗原,而对流电泳滴度又在1/16以上者,先用亲和层析法提取HBsAg,再通过挂有抗人血清蛋白抗体的亲和层析柱以除去残余的人血清蛋白。透析,并调整至1/2的对流电泳滴度,再用0.1％福尔马林在37℃作用48小时及4℃1周,以灭活未被发现的Dane颗粒。经过黑猩猩的实验接种2次,每次相隔1个月,观察5个月,证实肝功能无改变,血清内不出现HBsAg或抗HBc,而抗HBs则迅速升高,并维持在高水平,然后用于人体的自动免疫。共接种了96人,其中46人已观察5个月。接种前39人的HBsAg、抗HBs及抗HPc皆阴性,转氨酶及蛋白电泳正常,7人为低滴度的HBsAg携带者。46人中28人为血液透析病房工作人员,18人为肾功能不全患者,每人接种1毫升共2次,相隔1个月。另以16名工作人员和23名患者不接种作为对照。接种后
HBsAg positive blood donors were taken serum, selected which does not contain Dane particles or e antigen, and convection titers again in more than 1/16, the first affinity chromatography HBsAg extraction, and then linked by anti-human serum albumin antibody Affinity column to remove residual human serum albumin. Dialyzed, and adjusted to ½ of the titration by electrophoresis, and then inactivated 0.1% Dane pellets with formalin at 37 ° C for 48 hours and 4 ° C for 1 week. After chimpanzee experiment inoculation 2 times, every 1 month apart, observed for 5 months, confirmed that no change in liver function, serum HBsAg or anti-HBc does not appear, and anti-HBs rapidly increased and maintained at a high level, and then Autoimmunity for the human body. A total of 96 inoculated, of which 46 have been observed for 5 months. Before inoculation 39 people HBsAg, anti-HBs and anti-HPc were negative, normal aminotransferase and protein electrophoresis, 7 were low-titer HBsAg carriers. Of the 46 people, 28 were hemodialysis ward staff and 18 were patients with renal insufficiency. One person was vaccinated with 1 ml each for 2 times at intervals of 1 month. Another 16 staff and 23 patients were not vaccinated as a control. After inoculation