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应用抑凋亡基因bcl—2和P_(53)单克隆抗体,采用免疫组化LSAB法,对42例原发性肺癌进行研究,结果:肺癌bcl—2表达总阳性率为66.67％与肺炎性假瘤无显著差异(P>0.05),与正常支气管肺组织有非常显著差异(P<0.01),肺癌P_(53)表达总阳性率为50％与炎性假瘤组,正常支气管肺组织组差异非常显著(P<0.01);bcl—2和P_(53)表达在肺癌各组织类型间无 显著差异(P>0.05);但与肺癌的分化程度密切相关,P_(53)表达在肺癌有淋巴结转移组和无转移组差异显著(P<0.05);结果提示bcl—2表达是抑制细胞凋亡,P_(53)异常表达其主要作用是使细胞发生恶性转化增殖,两者在肺癌的发生、发展中起重要作用,结论是对肺癌原位检测bcl—2,P_(53)蛋白有助于肺癌的诊断和评估肺癌病人预后有重要意义。
Using anti-apoptotic gene bcl-2 and P53 monoclonal antibody, immunohistochemical LSAB method was used to study 42 cases of primary lung cancer. Results: The total positive rate of bcl-2 expression in lung cancer was 66.67% and pneumonia. There was no significant difference in sham tumors (P>0.05), and there was a significant difference between normal bronchopulmonary tissues (P<0.01). The total positive rate of P_(53) expression in lung cancer was 50% in inflammatory pseudotumor group and normal bronchopulmonary tissue group. The difference was very significant (P<0.01); bcl-2 and P53 expression were not significantly different among lung cancer tissue types (P>0.05); however, they were closely related to the degree of lung cancer differentiation. P53 expression in lung cancer There was a significant difference between lymph node metastasis group and non-metastasis group (P<0.05). The results suggested that bcl-2 expression was the inhibition of cell apoptosis. The main role of P_(53) abnormal expression was the malignant transformation and proliferation of the cells, both of which occurred in lung cancer. The important role of development in the development of lung cancer in situ detection of bcl-2, P53 protein is helpful for the diagnosis of lung cancer and the evaluation of prognosis of lung cancer patients.