丙二烯氧化合酶(allene oxide synthase, AOS)是茉莉酸脂加氧酶合成途径过程中的第一个酶。从冬小麦(Triticum aestivum L. cv. Jinghua No.3)克隆到了该酶的一个全长 cDNA 片段,其开放阅读框长约 1 410 bp,编码一约含470个氨基酸残基的多肽,推测其分子量为 51.9 kD。Southern 分析显示其在基因组中以 3 个拷贝的形式存在。Northern杂交分析表明该基因表达可被外源的茉莉酸诱导,诱导10 h时达到高峰,进一步的RNA 原位杂交表明该基因优先在幼叶中,尤其是在维管束附近的薄壁细胞中表达。同时,原位杂交还显示质膜钙通道的抑制剂 La3+并不能抑制外源茉莉酸诱导该区域 TaAOS 的表达。 Allel oxide synthase (AOS) is the first enzyme in the synthesis pathway of jasmonic acid-oxygenase. A full-length cDNA fragment of this enzyme was cloned from winter wheat (Triticum aestivum L. cv. Jinghua No.3). The open reading frame of this fragment was about 410 bp, encoding a polypeptide of 470 amino acid residues. 51.9 kD. Southern analysis shows that it is present in the genome in 3 copies. Northern blot analysis showed that the gene expression was induced by exogenous jasmonic acid and reached a peak at 10 h after induction. Further RNA in situ hybridization showed that the gene was preferentially expressed in young leaves, especially in parenchyma cells near vascular bundles . In situ hybridization also showed that La3 +, an inhibitor of plasma membrane calcium channels, did not inhibit the expression of TaAOS in the region induced by exogenous jasmonic acid.