【摘 要】
：
A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS). At the CAS
【机 构】
：
Key Laboratory of Ion Beam Bioengineering
【基金项目】
：
国家自然科学基金;The project of the National Major Technologies R&D Program of China for the 10th Five-Year Plan Period;国家优秀青年学者奖励计划
论文部分内容阅读
A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS). At the CAS-LIBB microbeam facility, we have developed protocols to place exact numbers of charged particles through nuclear centroids of cells, at defined positions in the cytoplasm relative to the nucleus, and through defined fractions of cells in a population. In this paper, we address the methods for nucleus, cytoplasm and bystander (either a single or an exact number of ions is delivered to a certain percentage of cells in a population to study the bystander effects of radiation) irradiation in detail from the precision of target finding and cell locating in the image analysis system. Moreover, for cells touching slightly in an image, a watershed method is used to separate these touching objects;after that, the number of objects in an image is counted accurately and the irradiation points are located precisely.
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