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目的研究海洋细菌NJ6-3-1次级代谢产物,以期得到有活性的先导化合物。方法采用硅胶柱色谱、凝胶柱色谱、HPLC等方法进行分离纯化,通过理化性质和波谱手段分析确定化合物的结构。结果从海洋细菌NJ6-3-1的乙酸乙酯萃取物中分离得到15个化合物,分别为环(色-脯)二肽(cyclo(Trp-Pro),1)、环(甘-脯)二肽(cyclo(Gly-Pro),2)、环(甘-苯丙)二肽(cyclo(Gly-Phe),3)、环(丙-苯丙)二肽(cylo(Ala-Phe),4)、环(酪-苯丙)二肽(cyclo(Tyr-Phe),5)、环(酪-脯)二肽(cy-clo(Tyr-Pro),6)、环(4-羟基-脯氨酸-苯丙氨酸)(cyclo(4-hydroxyl-Pro-Phe),7)、环(4-羟基-脯氨酸-亮氨酸)(cyclo(4-hydroxyl-Pro-Leu),8)、环(酪-亮)二肽(cyclo(Tyr-Leu),9)、环(丙-亮)二肽(cyclo(Ala-Leu),10)、环(甘-亮)二肽(cyclo(Gly-Leu),11)、环(丙-缬)二肽(cyclo(Ala-Val),12)、异光黄素(isolumichrome,13)、胸腺嘧啶(thymine,14)、尿嘧啶(uracil,15)。结论化合物1~15均为首次从海洋细菌NJ6-3-1次级代谢物中分离得到。
Objective To study the secondary metabolites of marine bacteria NJ6-3-1 in order to obtain active lead compounds. Methods The compounds were separated and purified by silica gel column chromatography, gel column chromatography and HPLC. The structures of the compounds were confirmed by physico-chemical properties and spectral analysis. Results Fifteen compounds were isolated from the ethyl acetate extract of marine bacteria NJ6-3-1 and were identified as cyclo (Trp-Pro), Cycloheximide Peptides (cyclo (Gly-Pro), 2), cyclo (Gly-Phe), 3), cylo (Ala-Phe) (Cyclo-Tyr-Phe) 5), cy-clo (Tyr-Pro) 6) Cyclo (4-hydroxyl-Pro-Phe), 7), cyclo (4-hydroxyl-Pro- Leu), 8 ), Cyclo (Tyr-Leu), 9), cyclo (Ala-Leu), 10) (Gly-Leu, 11), cyclo (Ala-Val), 12), isolumichrome (13), thymine (14), uracil , 15). Conclusion Compounds 1-15 were isolated from the secondary metabolites of marine bacteria NJ6-3-1 for the first time.