【摘 要】
：
Renal cell carcinoma is the most common cancer of the kidney,and resistant to traditional therapies.The aim of this study is to investigate the effects of hydro
【机 构】
：
Department of Urology,College of Chemistry,Department of Pathophysiology
论文部分内容阅读
Renal cell carcinoma is the most common cancer of the kidney,and resistant to traditional therapies.The aim of this study is to investigate the effects of hydroxyapatite nanoparticles on human renal cell carcinoma 786-0cells.Cell proliferation was assessed with an 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide(MTT)staining kit.The apoptosis assay was assessed with an FITC Annexin Ⅴ Apoptosis Detection Kit.Caspase-3 and caspase-12 were detected by immunocytochcmical staining and semi-quantitative RT-PCR.Cell wound healing assay was used to ensure cell motility.Matrigel invasion assay was analysed via transwell chambers.Our results showed that hydroxyapatite nanoparticles significantly reduced cell proliferation,invasion and induced apoptosis of 786-0 cells.The inhibiting action may have relation with up-regulated caspase-12,leading the cells to apoptosis.This study suggests that hydroxyapatite nanoparticles may be an effective and delivery system for renal cell carcinoma therapy.
其他文献
It is noted that there has been little research to compare volume-based and number-based soil particle size distributions(PSDs).Our objectives were to character
The authors study the existence of homoclinic type solutions for the following system of diffusion equations on R × RN:v{(e)tu-△xu+b·▽xu+au+V(t,x)v=Hv(t,x,u
Let D be the unit disc and H(D) be the set of all analytic functions on D. In [2], C. Cowen defined a space H = {f ∈ H(D): f(z) = ∞∑k=0 ak(z + 1)k, (A)z ∈ D
Kinetics model was developed for the mixed (steam and dry) reforming of methane, which is useful for the control of H2/CO ratio. The equilibrium constants of re
In this article, we introduce and examine some properties of new difference sequence spaces of fuzzy numbers defined using a sequence of modulus functions.
In this article, we deal with the uniqueness problems on meromorphic functions sharing two finite sets in an angular domain instead of the whole plane C. In par
Poly(4-vinylpyridinium wibromide) was prepared from poly(4-vinylpyridin) and used for the selective oxidation of a variety of sulfides to the corresponding sulf
A side-chain liquid crystalline ionomer(SLCI) was synthesized by grafting copolymerization of 4-(4-ethoxybenzoyloxy)-4'-allyloxybiphenyl and N-allyl-pyridium br
Two new concepts, the generalized support function and restricted chord function, both referring to a convex set, were introduced in [1]. General formulae to yi