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通过溶剂热法制备出高磁性的聚合物微球MnFe_2O_4,经正硅酸乙酯(TEOS)和3-氨基丙基三乙氧基硅烷(APTS)对微球表面进行改性修饰,制备出新型复合材料MnFe_2O_4@SiO_2-NH_2,并将其用于固定青霉素G酰化酶。在Si/Fe比为7 mmol/g、n(TEOS)∶n(APTS)=1∶1时,固定化酶PGA/MnFe_2O_4@SiO_2-NH_2在37℃下水解青霉素G钾合成6-氨基青霉烷酸,表观酶活为1 660 IU/g、载酶量为107.1 mg/g、比酶活为15.5 IU/mg、活性回收率为46.9%。经过6次重复使用,保留初始酶活的81.3%,在使用中固定化酶在磁场的作用下能够快速沉降与产物分离。
Highly magnetic polymer microspheres MnFe 2 O 4 were prepared by solvothermal method. The surface of the microspheres was modified by TEOS and APTS to prepare novel Composite material MnFe 2 O 4 @ SiO 2 -NH 2 was synthesized and immobilized on penicillin G acylase. The immobilized enzyme PGA / MnFe 2 O 4 @ SiO 2 -NH 2 hydrolyzed penicillin G at 37 ℃ to synthesize Penicillium 6-aminopeptidase at a Si / Fe ratio of 7 mmol / g and n (TEOS): n (APTS) Alkanoic acid had an apparent enzyme activity of 1 660 IU / g, an enzyme loading of 107.1 mg / g, a specific enzyme activity of 15.5 IU / mg and an activity recovery of 46.9%. After 6 times of repeated use, 81.3% of the initial enzyme activity is retained, and during use, the immobilized enzyme can rapidly settle down and separate from the product under the action of a magnetic field.