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利用辐射、花药培养及杀配子基因效应已创制出一系列小麦 -大赖草易位系。为在其中找出可能的纯合易位系、明确易位所涉及的相关染色体以及易位断裂点的确切位置 ,利用了已被定位于小麦 7个部分同源群染色体长、短两臂上的 67个探针进行了RFLP分析 ,结果鉴定出 3个纯合的易位系 :T1BL·7Lr#1S、T4BS·4BL - 7Lr#1S和T6AL·7Lr#1S。其中 ,易位系T1BL·7Lr #1S和T6AL·7Lr #1S中染色体 7Lr #1的断裂点位于标记MWG80 8和标记ABG476.1之间 ,而 1B和 6A染色体上的断裂点都在着丝粒附近。易位系T4BS·4BL - 7Lr#1S中染色体 7L #1的断裂点位于标记BCD349和标记CDO5 95之间 ,4B染色体断裂点则位于标记CDO5 4 1和标记PSR1 64之间的长臂上。
A series of wheat-Larvae translocations have been created using radiation, anther culture and gametocidal gene effects. In order to find out the possible homozygous translocation lines, the exact chromosome involved in the translocation and the exact position of the translocation breakpoint were utilized, and the long and short arms of the 7 homologous groups of wheat loci Of the 67 probes were analyzed by RFLP. As a result, 3 homozygous translots were identified: T1BL7Lr # 1S, T4BS4BL-7Lr # 1S and T6AL7Lr # 1S. Among them, the break point of chromosome 7Lr # 1 in the translocations T1BL · 7Lr # 1S and T6AL · 7Lr # 1S was located between the marker MWG80 8 and the marker ABG476.1, whereas the breakpoints on chromosomes 1B and 6A were all in the centromere nearby. The breakpoint of chromosome 7L # 1 in the translocation line T4BS · 4BL-7Lr # 1S was located between the marker BCD349 and the marker CDO5 95, while the point of chromosome 4B break was located on the long arm between the marker CDO5 4 1 and the marker PSR1 64.