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目的观察人胚胎生殖细胞移植于大鼠急性心肌梗死区后的存活率和增殖分化能力,探讨人胚胎生殖细胞异种异体移植的可行性。方法取5～9周人胚胎生殖脊细胞,分离、体外培养获得胚胎生殖细胞;用SD大鼠建立心肌梗死模型,分为对照组、移植组。分别在移植后1 d和1、2、4周处死大鼠,以抗人细胞核抗体MAB1281作为示踪剂,观察转录因子Nkx-2.5在移植大鼠心脏组织的阳性表达;分析人胚胎生殖细胞在宿主体内转化为心肌细胞的能力。结果成功建立大鼠心肌梗死模型;移植组心肌梗死区抗人细胞核抗体MAB1281和转录因子Nkx-2.5均呈阳性表达,对照组均为阴性表达。结论人胚胎生殖细胞植入心肌组织后,不仅在心肌内存活而且表现出向心肌细胞分化的特性。
Objective To observe the survival rate, proliferation and differentiation ability of human embryonic germ cells transplanted in acute myocardial infarction area in rats and to explore the feasibility of human embryo germ cell xenotransplantation. Methods Embryonic spine cells were obtained from 5 to 9 weeks, isolated and cultured in vitro to obtain embryonic germ cells. The model of myocardial infarction was established in SD rats and divided into control group and transplantation group. The rats were sacrificed on day 1, 2, and 4 after transplantation, respectively. The anti-human nuclear antibody MAB1281 was used as a tracer to observe the positive expression of transcription factor Nkx-2.5 in the transplanted rat heart. The ability of the host to convert into cardiomyocytes. Results The model of myocardial infarction in rats was successfully established. The expression of anti-human nuclear antibody MAB1281 and Nkx-2.5 in the myocardial infarction area were all positive in the transplantation group, but negative in the control group. Conclusions After human embryonic germ cells are implanted into myocardial tissue, they not only survive in the myocardium but also show the characteristics of differentiating into cardiomyocytes.