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目的：为探索性构建重组人白细胞介素６－绿脓杆菌外毒素融合蛋白（ＩＬ６－ＰＥ４０）以选择性杀伤高表达ＩＬ６受体（ＩＬ６Ｒ）的白血病细胞，本研究试图从人急性早幼粒细胞白血病细胞株（ＨＬ－６０）中克隆Ｎ－末端缺失２４个氨基酸的人ＩＬ６基因（ＩＬ６ｃＤＮＡ）并构建含此基因的重组质粒。方法：根据ＩＬ６基因序列设计合成可扩增ＩＬ６ｃＤＮＡ的特异性引物；利用基因重组技术，构建含ＩＬ６基因的重组质粒ｐＵＣ－ＩＬ６。结果与结论：本文首次从ＨＬ－６０中扩增出预期４８０ｂｐ的ＩＬ６ｃＤＮＡ，将其克隆至ｐＵＣ１８质粒中，命名为ｐＵＣ－ＩＬ６，并经ＥｃｏＲＩ／ＢａｍＨＩ双酶切电泳及序列分析鉴定加以确证，为进一步构建重组人ＩＬ６－ＰＥ４０奠定了坚实基础。
OBJECTIVE: To construct a recombinant human interleukin 6-Pseudomonas exotoxin fusion protein (IL6-PE40) for the selective killing of leukemia cells that highly express the IL6 receptor (IL6R). This study was attempted from human acute promyelosomes. A human IL6 gene (IL6 cDNA) having a 24 amino acid deletion at the N-terminus was cloned from a cell leukemia cell line (HL-60) and a recombinant plasmid containing this gene was constructed. Methods: According to the IL6 gene sequence, we designed and synthesized specific primers that can amplify IL6 cDNA. Using gene recombination technology, the recombinant plasmid pUC-IL6 containing IL6 gene was constructed. RESULTS AND CONCLUSION: This study first amplified the expected 480 bp IL6 cDNA from HL-60 and cloned it into pUC18 plasmid named pUC-IL6. The result was confirmed by EcoRI/BamHI double enzyme electrophoresis and sequence analysis. The establishment of recombinant human IL6-PE40 laid a solid foundation.