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[目的]研究野大麦盐胁迫rbcS基因的克隆及序列分析。[方法]以盐胁迫下的野大麦幼嫩叶片为试材,根据GenBank中小麦和大麦rbcS基因核酸序列的同源性设计引物,对野大麦基因组DNA进行PCR扩增、回收、连接、转化及测序。[结果]在野大麦的基因组中克隆了2个大小不同的rbcS基因序列rbcS1和rbcS2,长度分别为1252和908bp。rbcS1和rbcS2均由2个外显子和1个内含子组成,外显子长度相等,编码序列为525bp,同源性为96%,编码174个氨基酸;rbcS1和rbcS2内含子大小不同,分别为448和107bp。[结论]该研究为进一步探讨rbcS基因在野大麦耐盐机制中的分子机制奠定了基础。
[Objective] The research aimed to study the cloning and sequence analysis of rbcS gene under salt stress of wild barley. [Method] With the young leaves of wild barley under salt stress as test material, primers were designed according to the homology of rbcS gene of wheat and barley in GenBank to amplify, recover, ligate and transform genomic DNA of wild barley Sequencing. [Result] Two rbcS gene sequences rbcS1 and rbcS2 of different sizes were cloned in the genome of wild barley, and their lengths were 1252 and 908 bp, respectively. Both rbcS1 and rbcS2 are composed of two exons and one intron. The length of exon is equal, the coding sequence is 525 bp, the homology is 96%, encoding 174 amino acids; rbcS1 and rbcS2 intron are different in size, Respectively 448 and 107bp. [Conclusion] This study lays the foundation for further exploring the molecular mechanism of rbcS gene in the mechanism of salt tolerance in barley.