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目的 :探讨急性应激状态下脑内一氧化氮 (NO)含量和一氧化氮合酶 (NOS)活性的变化及意义。方法 :采用放免法测定强迫游泳应激后 1、 3小时的大鼠额叶、海马、中脑和下丘脑内NO含量和NOS活性。结果 :应激结束后 1小时 ,海马和下丘脑内NO含量和NOS活性均显著性增高 (t分别为 2 3 2、 2 61,P均 <0 0 5 )。应激结束后 3小时 ,额叶、海马、中脑和下丘脑内NO含量均显著性增高 (t分别为 3 5 7、4 2 6、 3 88、 4 93 ,P均 <0 0 1) ,NOS活性均显著性增高 (t分别为 2 3 2、 2 61,P均 <0 0 5 )。结论 :急性应激状态下 ,各脑区的NOS活性增加 ,产生的NO可能介导了神经毒性作用
Objective: To investigate the changes of brain nitric oxide (NO) content and nitric oxide synthase (NOS) activity in acute stress and its significance. Methods: The contents of NO and NOS in rat frontal lobe, hippocampus, midbrain and hypothalamus were measured by radioimmunoassay at 1 and 3 hours after forced swimming stress. Results: The levels of NO and NOS in the hippocampus and hypothalamus were significantly increased 1 hour after the end of stress (t 2 3 2, 2 61, P 0 05 respectively). Three hours after the end of stress, NO content in the frontal lobe, hippocampus, midbrain and hypothalamus were significantly increased (t = 357, 4262, 388, and 493, respectively, P <0.01) NOS activity were significantly increased (t respectively 2 3 2, 2 61, P all <0 0 5). CONCLUSIONS: Under acute stress conditions, NOS activity increases in all brain regions and NO production may mediate neurotoxic effects