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目的探讨大肠癌石蜡包埋组织中K-ras基因突变状态及其与年龄、性别、病理组织学分型和分化程度的关系。方法选用ADx-ARMS法检测84例大肠癌石蜡组织中K-ras基因7个突变热点。结果 82例大肠癌样本中共发现K-ras基因突变30例,突变率为36.6%。K-ras基因突变与患者年龄、性别、大肠腺癌组织学类型及分化程度无关(P>0.05)。结论 K-ras基因在大肠癌中的突变率与肿瘤类型、分化程度、患者年龄及性别无关。
Objective To investigate the mutation status of K-ras gene in paraffin-embedded tissues of colorectal cancer and its relationship with age, sex, histopathological type and differentiation. Methods ADX-ARMS method was used to detect seven mutations of K-ras gene in 84 cases of colorectal cancer paraffin tissues. Results Thirty cases of K-ras gene mutation were found in 82 cases of colorectal cancer with a mutation rate of 36.6%. The mutation of K-ras was not associated with the age, sex, histological type and differentiation of adenocarcinoma (P> 0.05). Conclusion The mutation rate of K-ras gene in colorectal cancer is not related to tumor type, differentiation degree, patient’s age and gender.