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目的了解方形黄鼠蚤阿尔泰亚种带菌越冬后菌栓形成的客观水平。方法2006年9月28日将饥饿3d的方形黄鼠蚤阿尔泰亚种,置感染鼠疫菌而濒死的小鼠体吸血,剔出未吸血蚤,将感染的方形黄鼠蚤413只置于温度2～4℃,相对湿度75%～85%的冷藏箱中。2007年4月6日将蚤从冷藏箱取出,用小鼠全天喂饲吸血1次。此后置于温度20～22℃,相对湿度75%～85%温箱中,隔日用健康小鼠供血0.5～1h,镜下观察菌栓形成情况。结果经过一个冬眠期共存活235只方形黄鼠蚤,形成菌栓蚤9匹,菌栓率3.83%(9/235)。结论方形黄鼠蚤带菌越冬后菌栓形成率明显低于黄鼠活动期菌栓蚤的形成率(13.8%),这可能与蚤在不利环境下出现滞育现象和一部分蚤已摆脱鼠疫菌或带菌量减少,低于102～104有关。
Objective To understand the objective level of bacteremia after overwintering of Altaia species of Cartilaginous. METHODS: On September 28, 2006, starved darts of Artemisia fontanella, which were starved for 3d, were drowned in mice that were infected with Yersinia pestis, 2 ~ 4 ℃, relative humidity 75% ~ 85% of the freezer. April 6, 2007 The fleas were removed from the freezer and the mice were fed a single vampire throughout the day. Thereafter placed in a temperature of 20 ~ 22 ℃, relative humidity 75% ~ 85% incubator, with healthy mice every other day for 0.5 ~ 1h, microscopic observation of the formation of bacteria plug. Results After living together for 235 days, a total of 235 square-shaped shrimp fleas were found, 9 of which were caused by the bacterial plug, with a rate of 3.83% (9/235). Conclusions The rate of formation of mycelial plug after overwintering was significantly lower than that of T. gondii (13.8%) during the overwintering period, which may be related to the diapause of fleas in unfavorable environment and part of the fleas had rid themselves of Yersinia pestis With less bacteria, less than 102 ~ 104.