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构建了含有CMV启动子调控的HSV－tk基因的重组质粒pAdCMVtk，将其与缺陷型腺病毒Ad5大框架pJM17一起共转染病毒包装细胞293细胞，获得有感染能力但复制缺陷的重组腺病毒AdCMVtk，以其感染大鼠C5脑胶质瘤细胞后用PCR方法证实HSV-tk基因能被腺病毒有效转入靶细胞。用带报告基因LacZ的腺病毒感染大鼠C5脑胶质瘤细胞，经x-gal染色，证明腺病毒载体的基因转移效率可达100％。
The recombinant plasmid pAdCMVtk containing HSV-tk gene regulated by CMV promoter was constructed and co-transfected with the defective adenovirus Ad5 large frame pJM17 into 293 cells of virus-packaged cells to obtain the recombinant adenovirus AdCMVtk , With its infection of rat C5 glioma cells confirmed by PCR PCR HSV-tk gene can be effectively transferred by adenovirus target cells. Rat C5 glioma cells were infected with the adenovirus carrying the reporter gene LacZ and confirmed by x-gal staining that the gene transfer efficiency of the adenovirus vector was up to 100%.