论文部分内容阅读
使用 DNS— cl( Dimethylamino-naphthylane-5-sulfony chloride)荧光试剂 ,标记氨基酸成 DNS— AAs( DNS_ Amino Acids) ,然后采用聚酰胺薄膜层析 ,制成具有 2 0余种 DNS— AAs的荧光薄膜层析图谱 ,选择其中所需要检测的氨基酸 ,分别经洗脱后在日本岛津 RF— 51 0荧光分光光度计上 ,进行定量检测 ,分析了 3 6例慢性肾炎患者 ,1 8例尿毒症患者血浆中 1 2种游离氨基酸和 3 5例慢性肾炎患者尿液中 1 3种游离氨基酸 ,分别与 2 9例正常人血浆和3 0例正常人尿液中游离氨基酸进行了比较 ,以此探讨肾脏疾病体液氨基酸模式的同时提供氨基酸治疗肾脏疾病的科学依据。
Amino acids were labeled with DNS-AAs (DNS_Amino Acids) using a DNS-cl (Dimethylamino-naphthylane-5-sulfony chloride) fluorescent reagent and then a polyamide film was used to make a fluorescent film with more than 20 DNS- Chromatogram, select the amino acids needed for detection, respectively, after elution in Japan Shimadzu RF-51 0 fluorescence spectrophotometer for quantitative detection of 36 cases of chronic nephritis patients, 18 cases of uremic patients 13 free amino acids in plasma and 13 free amino acids in urine from 35 patients with chronic nephritis were compared with the free amino acids in urine of 29 normal individuals and 30 normal individuals respectively, Diseases Amino acid patterns of body fluids provide the scientific basis for the treatment of kidney disease with amino acids.