在原有PCR(聚合酶链反应)技术的基础上,本组采用微量全血,经PCR扩增,可直接检测载脂蛋白基因突变,获得了很好的效果。此方法仅需极微量全血,操作简便,灵敏度高,不需特殊设备,可用于大规模地筛选突变基因及临床检验。 将全血(12μl即可达到检测目的)于室温干燥条件下固定在一洁净载体上。样品可保存于室温、4℃ In the original PCR (Polymerase Chain Reaction) technology, based on the use of micro-whole blood in this group, amplified by PCR, apolipoprotein gene can be directly detected mutations, and achieved good results. This method requires only a trace amount of whole blood, easy operation, high sensitivity, without special equipment, can be used for large-scale screening of mutant genes and clinical tests. Whole blood (12 μl for testing purposes) is immobilized on a clean carrier at room temperature in dry conditions. Samples can be stored at room temperature, 4 ℃