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目的:建立同时测定丹参中2种水溶性成分和2种脂溶性成分的方法。方法:HPLC-DAD法,采用Agilent Zorbax TC C18柱(4.6mm×250mm,5μm),以甲醇:2%醋酸为流动相进行梯度洗脱:0～15min,30%B～40%B;15～20min,40%B～60%B;20～25min,60%B～90%B;25～40min,90%B。检测波长为281nm,柱温:35℃。结果:迷迭香酸、丹酚酸B、隐丹参酮、丹参酮ⅡA浓度分别在3.76～120.20μg·ml-1(r=0.9999)、34.20～1094.5μg·ml-1(r=0.9999)、0.64～20.32μg·ml-1(r=0.9999)、1.02～32.72μg·ml-1(r=0.9996)范围内呈良好的线性关系。4种成分精密度试验RSD<1%。48h内稳定性RSD<1%。加样回收率为99.72%～100.63%。结论:该含量测定方法简便,分离效果好,能同时测定丹参中迷迭香酸、丹酚酸B、隐丹参酮及丹参酮ⅡA四种有效成分的含量,结果准确可靠。
Objective: To establish a method for simultaneous determination of two water-soluble components and two fat-soluble components in Salvia miltiorrhiza. Method: HPLC-DAD method, using Agilent Zorbax TC C18 column (4.6mm × 250mm, 5μm), gradient elution with methanol: 2% acetic acid as the mobile phase: 0 ~ 15min, 30% B ~ 40% B; 15 ~ 20 min, 40% B to 60% B; 20 to 25 min, 60% B to 90% B; 25 to 40 min, 90% B. The detection wavelength was 281 nm, and the column temperature was 35°C. Results: The concentrations of rosmarinic acid, salvianolic acid B, cryptotanshinone, and tanshinone IIA were 3.76-120.20 μg·ml-1 (r=0.9999), 34.20-1094.5 μg·ml-1 (r=0.9999), 0.64～, respectively. There was a good linear relationship between 20.32 μg·ml-1 (r=0.9999) and 1.02 to 32.72 μg·ml-1 (r=0.9996). Four component precision tests RSD < 1%. 48h stability RSD <1%. The sample recovery rate was 99.72% to 100.63%. Conclusion: The determination method is simple, and the separation effect is good. It can be used to determine the contents of four active ingredients of rosmarinic acid, salvianolic acid B, cryptotanshinone and tanshinone IIA in Salvia miltiorrhiza. The results are accurate and reliable.