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目的:探讨糖克煎剂对糖尿病肾病大鼠肾脏miR-192信号通路的影响。方法:大鼠一次性尾静脉注射链脲佐菌素30mg/kg建立糖尿病模型,随机分为模型组、糖克煎剂4.5g/kg预防组、糖克煎剂4.5g/kg、18g/kg剂量组、福辛普利钠片组(0.9mg/kg),选取同龄大鼠作为正常对照组。测定血糖、24h尿蛋白、左肾重、肾重/体重比、BUN、Scr、TC、TG水平,HE染色观察肾脏组织病理变化,QRT-PCR法检测肾组织TGF-β1、miR-192、SIP1、Col1a2 m RNA的表达,Western-blot法检测肾脏中TGF-β1、SIP1、Collagen I蛋白的表达。结果:与模型组相比,糖克煎剂4.5g/kg预防组、糖克煎剂4.5g/kg、18g/kg剂量组和福辛普利钠片组24h尿蛋白量、左肾重、肾重/体重比、BUN、Scr、TC、TG显著降低,肾小球基底膜增厚、系膜增生等病理损害得到改善,肾脏TGF-β1、miR-192、Col1a2m RNA表达减少,SIP1m RNA及蛋白表达增高,肾脏TGF-β1、Collagen I蛋白表达降低,糖克煎剂18g/kg剂量组效果更好。结论:糖克煎剂能够治疗糖尿病肾病,其保护肾脏的机制可能与抑制miR-192信号通路相关。
Objective: To investigate the effect of Tang Ke decoction on the kidney miR-192 signal pathway in diabetic nephropathy rats. Methods: Diabetic rats were induced by streptozotocin (30mg / kg) once a day and were randomly divided into 4 groups: model group, Dose group, fosinopril sodium tablets group (0.9mg / kg), select the same age rats as the normal control group. The levels of blood glucose, 24h urinary protein, left kidney weight, kidney weight / body weight ratio, BUN, Scr, TC, TG levels were measured. The pathological changes of kidney were observed by HE staining. The expressions of TGF-β1, , Col1a2 m RNA expression, Western-blot assay of TGF-β1, SIP1, Collagen I protein expression. Results: Compared with model group, the contents of 24 h urinary protein, left kidney weight, blood sugar, blood sugar and blood sugar of 4.5 g / kg prevention group, 4.5 g / kg, 18 g / Kidney weight / body weight ratio, BUN, Scr, TC, TG were significantly decreased, glomerular basement membrane thickening, mesangial proliferation and other pathological lesions improved, the expression of TGF-β1, miR-192, Col1a2mRNA decreased, SIP1mRNA and The expression of TGF-β1 and Collagen I in kidney were decreased, and the effect of Cork decoction 18g / kg was better. Conclusion: Tang Ke decoction can treat diabetic nephropathy, and its mechanism of protecting kidneys may be related to the inhibition of miR-192 signaling pathway.