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目的探讨形态学检查联合流式细胞术(FCM)对淋巴瘤骨髓浸润的临床应用。方法对77例淋巴瘤患者的骨髓标本行FCM免疫表型、涂片及活检,观察骨髓受累率,并初步评价其临床价值。结果 11例霍奇金淋巴瘤(HL)切片法仅发现1例骨髓受累;66例非霍奇金淋巴瘤(NHL)中,FCM检出41例,涂片法检出8例,切片法检出19例。FCM发现的41例中,18例为早期浸润(瘤细胞<5%);20例为小细胞淋巴瘤(SLL);2例为浆细胞性淋巴瘤,2例为滤泡性淋巴瘤,1例同时表达T、B细胞抗原。结论形态学联合FCM检查提高了骨髓受累的检出率,免疫表型对早期浸润和SLL有重要诊断价值。
Objective To investigate the clinical application of morphological examination combined with flow cytometry (FCM) in infiltration of bone marrow of lymphoma. Methods The bone marrow samples from 77 patients with lymphoma were immunophenotype, smear and biopsy. The bone marrow involvement rate was observed and the clinical value was preliminary evaluated. Results Only 11 cases of bone marrow involvement were found in 11 cases of Hodgkin ’s lymphoma (HL). In 66 cases of non - Hodgkin’ s lymphoma (NHL), 41 cases were detected by FCM, 8 cases were smear method, Out of 19 cases. Of the 41 patients identified by FCM, 18 were early infiltrating (<5% tumor cells); 20 were small cell lymphoma (SLL); 2 were plasmacytoid lymphoma, 2 were follicular lymphoma, 1 Example simultaneous expression of T, B cell antigen. Conclusion Morphology combined with FCM improves the detection rate of bone marrow involvement. Immunophenotype has important diagnostic value for early infiltration and SLL.