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血清溶菌酶测定是一种有效和经济的诊断腹腔内脓肿方法.溶菌酶为分子量14000的蛋白,位于吞噬细胞、粒细胞和单核细胞的溶酶体内.脓肿形成时必然伴有血清溶菌酶增高,作者进行了动物实验和临床观察,溶菌酶分析则用可见光分光光度计的标准比浊法测定.动物实验 取55只CDI雄鼠,20只作为对照.35只制作腹腔脓肿模型,即用盲肠结扎和穿刺的CLP技术.脓肿直径约为0.2cm～2.Ocm.结果发现脓肿组的溶菌酶活力比对照组增高44%(P<0.001),其中CLP后7天、14天和21天分别增加33%、
Serum lysozyme assay is an effective and economical method for the diagnosis of intraperitoneal abscess.Lysozyme is a protein with a molecular weight of 14000 located in the lysosomes of phagocytes, granulocytes and mononuclear cells.If the abscess formation is accompanied by an increase of serum lysozyme , The author conducted animal experiments and clinical observation, lysozyme analysis using visible spectrophotometer standard nephelometry determination of animal experiments take 55 CDI male mice, 20 as a control .35 only made intraperitoneal abscess model, that is, with the cecum Ligation and puncture of CLP technology abscess diameter of about 0.2cm ~ 2.Ocm results showed that the abscess group lysozyme activity 44% higher than the control group (P <0.001), which were 7 days after CLP, 14 days and 21 days respectively Increase by 33%