作者从盾叶鬼臼Podophyllum peltatum WangWilcom的根茎中分离出内生真菌Phialocephala fortinii(一种食用菌)PPE5和PPE7株。经过4周的培养,这2株真菌在肉汤培养基中可产生鬼臼毒素。将采集的盾叶鬼臼的叶、叶柄、根茎和根等部位部分进行表面灭菌后冲洗。然后把这些部位切成1c The authors isolated endophytic fungi Phialocephala fortinii (an edible fungus) PPE5 and PPE7 from the roots of Podophyllum peltatum WangWilcom. After 4 weeks of culture, the 2 fungi produced podophyllotoxin in the broth. The collected leaves, petioles, rhizomes, roots, and other parts of the shield leaf were treated with surface sterilization and rinsed. Then cut these parts into 1c