论文部分内容阅读
我们从基因库中筛选出乙型肝炎病毒（HBV）高度保守序列片段,通过分子克隆操作,用芯片点样仪点到特制的玻片上,制成乙型肝炎基因诊断芯片,检测了99例乙型肝炎肝硬化患者肝组织标本,同时用免疫组织化学法、原位分子杂交法检测乙型肝炎核心抗原（HBcAg）和HBV DNA。1.资料与方法:（1）芯片制备:玻片购自Sigma公司,HBV DNA靶基因和对照植物基因由博华公司提供,用通用引物进行PCR扩增,PCR产物用Cartesia7500点样仪进行点样制成芯片。（2）标本处理和DNA提取:采集本院病理科1995～2000年乙型肝炎后肝硬化肝活检
We screened out highly conserved sequence fragments of hepatitis B virus (HBV) from the gene pool. Through the molecular cloning operation, we spotted the sample onto a special glass slide to make a Hepatitis B gene diagnostic chip, and detected 99 cases Hepatic cirrhosis patients with liver tissue samples, at the same time by immunohistochemistry, in situ hybridization detection of hepatitis B core antigen (HBcAg) and HBV DNA. 1. Materials and Methods: (1) Preparation of chips: Slides were purchased from Sigma Company. The HBV DNA target genes and control plant genes were provided by Bohua Company. PCR was performed by universal primers. PCR products were spotted with Cartesia 7500 spotter Sample made of chips. (2) specimen processing and DNA extraction: collection of hospital pathology 1995-2000 liver cirrhosis after liver biopsy