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用ＲＰ－ＨＰＬＣ测定人血浆中洛伐他汀浓度。以甲醇－水（８３∶１７，Ｖ／Ｖ）为流动相，色谱柱采用ＨＹＰＥＲＳＩＬＢＤＳ－Ｃ１８（５μｍ）不锈钢柱，紫外２３７ｎｍ波长检测。血浆样品经环己烷－异丙醇（９５∶５）萃取浓缩后进样测定。洛伐他汀浓度在２．５～８０ｎｇ／ｍｌ范围内线性良好，ｒ＝０．９９６８。测定含洛伐他汀２０．０ｎｇ／ｍｌ的血浆样品，其日内（ｎ＝７）及日间（ｎ＝７）的ＲＳＤ分别为９．８％和８．５％。回收率平均为（１０１．３±５．５）％。测定了１０名健康志愿者单次ｐｏ洛伐他汀片剂８０ｍｇ后不同时间的血药浓度，计算了相应的药动学参数。
The concentration of lovastatin in human plasma was determined by RP-HPLC. The mobile phase was methanol-water (83:17, V / V). The column was detected on a HYPERSILBDS-C18 (5μm) stainless steel column with a UV detection wavelength of 237nm. Plasma samples were extracted by cyclohexane-isopropanol (95: 5) and concentrated before injection. Lovastatin concentrations ranged from 2.5 to 80 ng / ml with good linearity, r = 0.9968. Plasma samples containing lovastatin 20.0 ng / ml were assayed with RSDs of 9.8% and 8.5% during the day (n = 7) and during the day (n = 7), respectively. The average recoveries were (101.3 ± 5.5)%. The pharmacokinetic parameters of 10 healthy volunteers were determined at different times after 80 mg po po lovastatin tablet.