目前,一些研究者正在探讨霍乱毒素B亚单位(Ctx B)作为粘膜免疫佐剂的应用潜力。有证据表明将抗原与Ctx B结合(而非简单地混合)后能提高抗原的口服免疫原性。1990年作者曾构建基因工程Ctx B融合蛋白,发现短肽段可以融合于Ctx B的N末端而对Ctx B的结构和功能很少影响。在本研究中,作者将编码8、12和24个 Currently, some researchers are exploring the potential of cholera toxin B subunit (Ctx B) as mucosal immune adjuvant. There is evidence that increasing the oral immunogenicity of an antigen after antigen binding to Ctx B (rather than simply mixing). In 1990, the authors constructed a genetically engineered CtxB fusion protein and found that the short peptide fragment can be fused to the N-terminus of CtxB with little effect on the structure and function of CtxB. In this study, the authors will encode 8, 12 and 24