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目的:研究雄性大鼠去势1至4个月后,血管活性物质对离体动脉作用的改变.方法:采用双侧睾丸切除的雄性大鼠,分为假手术组(SHAM)、去势组(ORDX)和雄激素替代组(TP).于替代后 1、2及 4个月时,取胸主动脉、肺动脉及尾动脉,进行离体血管功能实验,观察对去甲肾上腺素(NE)、降钙素基因相关肽(CGRP)及乙酰胆碱(ACh)的反应.结果:去势 1个月后,NE、ACh和 CGRP引起的大鼠离体动脉收缩和舒张反应无明显改变,仅在主动脉,TP组CGRP的舒张效应曲线明显左移;2个月后,NE、ACh、CGRP引起的大鼠离体动脉收缩和舒张反应无明显改变;4个月后,对 CGRP和 ACh的舒张反应无明显改变,但ORDX组对NE引起的主动脉收缩反应曲线左移,替代后恢复.结论:相对于雌激素,雄激素对血管的保护作用较弱,其机制可能是通过抑制血管的收缩反应.
OBJECTIVE: To study the effect of vasoactive substances on isolated arteries in rats from 1 to 4 months after ovariectomy.Methods: Male rats were divided into sham operation group (SHAM) (ORDX) and androgen replacement group (TP) .At 1, 2 and 4 months after replacement, the thoracic aorta, pulmonary artery and caudal artery were taken for in vitro vascular function test to observe the effects of norepinephrine (CGRP) and acetylcholine (ACh) .Results: After 1 month of ovariectomy, there was no significant change in isolated rat arterial systolic and diastolic responses induced by NE, ACh and CGRP, but only in the aorta , The diastolic effect curve of CGRP in TP group shifted to the left significantly. After 2 months, there was no significant change in the contractile and diastolic responses of rat isolated arteries induced by NE, ACh and CGRP. After 4 months, the relaxation responses to CGRP and ACh However, the ORDX group shifted to the left of the aortic contractile response induced by NE, and then recovered after replacement.Conclusion: Compared with estrogen, the protective effect of androgens on blood vessels is weak, which may be due to the inhibition of vascular contractile response.