论文部分内容阅读
目的探讨阿藿烯对血栓形成的影响及其机制。方法将SD大鼠随机分为正常对照组、阳性对照组、阿藿烯低、中、高剂量共5组,灌胃7d后采用动-静脉旁路血栓形成法制作大鼠血栓模型,测量血栓湿重,检测血清P-选择素(Ps)和血管细胞黏附分子-1(VCAM-1)水平;并以花生四烯酸(AA)作诱导剂,观察阿藿烯对兔血小板聚集的作用。结果低、中、高剂量组阿藿烯均可显著抑制血栓形成(P<0.01或P<0.05),显著降低血小板最大聚集率及血清Ps水平(P<0.01);中、高剂量组阿藿烯还可显著减少VCAM-1含量(P<0.01)。结论阿藿烯能够抑制大鼠动-静脉旁路的血栓形成,其机制与减少血小板聚集,抑制VCAM-1和Ps的表达有关。
Objective To investigate the effect of acetonide on thrombosis and its mechanism. Methods Sprague-Dawley rats were randomly divided into normal control group, positive control group, low, medium and high dose of acetonide for 5 days. After 7 days of intragastric administration, rat thrombosis model was established by arterial-venous bypass thrombosis. Thrombus Wet weight, serum P-selectin (Ps) and vascular cell adhesion molecule-1 (VCAM-1) levels were measured. Arachidonic acid (AA) was used as an inducer to observe the effect of acetonide on platelet aggregation in rabbits. Results Alloxene significantly inhibited thrombosis (P <0.01 or P <0.05), significantly reduced the maximum platelet aggregation rate and serum Ps level (P <0.01) in low, medium and high dose groups, Alkene also significantly reduced VCAM-1 levels (P <0.01). Conclusions Alloxene can inhibit the thrombosis of rat arteriovenous venous bypass. Its mechanism is related to reducing platelet aggregation and inhibiting the expression of VCAM-1 and Ps.